Discovery of genes involved in infertility

Elucidation of the cell division mechanisms necessary for germ cell formation

Most cells in the tissues of most organisms proliferate through somatic cell division (mitosis). This is a continuous cycle where a single cell doubles its genetic information (chromosomes) and divides equally to create two copies of the original cell. In contrast, germ (egg and sperm) cells are produced through a special type of cell division called meiosis, which takes place in the gonads. This process begins like normal mitosis but switches after some time to create four genetically dissimilar germ cells that have half the original cell’s genetic material. The mechanism that causes this switch has been a longstanding research problem and controlling it is an important, yet challenging, global issue in reproductive medicine.

Recently, a group of researchers from Kumamoto University in Japan set out to assess the proteins involved in meiosis to clarify its development. Using mass spectrometry, the group was able to identify a gene that acts as a meiosis on-switch, labeling it “Meiosin” (for meiosis initiator). The Meiosin gene has the extremely rare property of becoming activated only at a specific time–just before the beginning of meiosis in the gonads. In animal experiments, the researchers found that both male and female mice became infertile if the Meiosin gene was artificially inhibited. Further analysis of the mouse male and female gonads clarified that the gene plays an essential role in meiosis activation. The Meiosin gene acts as a control tower that simultaneously switches on hundreds of genes for germ cell formation, and appears to be involved with infertility. The group expects that the discovery will be a big step forward for reproductive medicine.

There are many cases of human infertility where the cause is unknown; this finding potentially reveals a new pathology. Even though these experiments were performed on animal models, the Meiosin gene is present in humans. Abnormal meiosis in the eggs of older women can cause chromosomal abnormalities resulting in miscarriage or Down syndrome. And in societies with increased age of pregnancy, therapies developed from this research could ensure meiosis quality and decrease the instances of these complications.

“Our work shows that the Meiosin gene is the switch that turns on meiosis, the special type of cell division that creates eggs and sperm, and this includes the turning-on of hundreds of other genes in the process. However, the functions of those genes have not yet been fully elucidated,” said Associate Professor Kei-Ichiro Ishiguro of the Institute of Molecular Embryology and Genetics at Kumamoto University. “We were quite surprised to find so many genes with undefined functions lying dormant in this study. We have high expectations that the processes involved in germ cell formation will be greatly clarified as the role each of these genes plays is discovered. If it eventually becomes possible to control meiosis, the benefits would be far-reaching for reproductive medicine, agricultural production, and even assisting rare species reproduction.”

This research was posted online in “Developmental Cell” on 6 February 2020.

Paper Information

  • Authors: Ishiguro, Kei-ichiro, Kumi Matsuura, Naoki Tani, Naoki Takeda, Shingo Usuki, Mariko Yamane, Michihiko Sugimoto, et al.
  • Title: MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells
  • Journal: Developmental Cell
  • DOI: https://doi.org/10.1016/j.devcel.2020.01.010

Funding

  • 1) Japanese Ministry of Education Culture Sports Science and Technology (MEXT) – Grants-in-Aid for Scientific Research (KAKENHI),
  • 2) National Institute of Genetics – Joint Research and Research Meetings (NIG-JOINT),
  • 3) IMEG Research Center for Developmental Medicine (Kumamoto University) – Program of the Joint Usage,
  • 4) Takeda Science Foundation, 5) Yamada Science Foundation, 6) Ichiro Kanehara Foundation for Medical Science and Medical Care

Numbers

  • 1) JP19H05748; JP16H01257; JP16H01221; JP17H03634; JP18K19304; JP19H05245; JP19H05743; JP16H06276,
  • 2) 44A2017; 40A2018; 32A2019,
  • 3) NA, 4) NA, 5) NA, 6) NA

Medias

  • CREDIT: Assoc. Prof. Kei-ichiro Ishiguro
  • USAGE RESTRICTIONS: This image may only be used in conjunction with the accompanying release, or stories written about the work described in the release with reference to the original work.
Media 1: The Meiosin gene initiates the switch from mitosis to meiosis
In germ cell development, the newly discovered Meiosin gene triggers the change from mitosis to meiosis. It turns on hundreds of other genes that appear to work together to form sperm and egg cells in animals.
Media 2: Inhibition of the Meiosin gene causes animal specimens to become infertile
Researchers from Kumamoto University (Japan) performed genome editing experiments to verify that the Meiosin gene was indeed the trigger for meiosis. When they removed the gene in a murine experimental model, meiosis did not begin and germ cells were not produced rendering the animals infertile.
Media 3: Schematic of meiosis being activated by the Meiosin gene during somatic cell division (mitosis)
Mitosis is the main cell reproduction process in a living organism’s tissues, and meiosis is the process that induces sex cell formation in the gonads of organisms that reproduce sexually. The Meiosin gene, at the crossroads between the mitotic and meiotic cycles, gives the signal that initiates meiosis. After the signal is given, the Meiosin protein binds to multiple genes and issues a command to activate the meiotic process.

Media Contact

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